In vitro Viability and Fertilizing Capacity of Guinea Fowl Spermatozoa : Reproductive Biology

概要

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In vitro viability and fertilizing capacity of guinea fowl spermatozoa were examined by the method of storing semen at 0℃ (in ice). Freshly ejaculated undiluted guinea fowl spermatozoa survived for only three days, in clear contrast to those which were diluted with a phosphate buffer and retained their motility for up to ten days. The number of abnormal spermatozoa increased considerably during the period of preservation of undiluted semen. A relatively high fertility was obtained from both undiluted and diluted semen when they were inseminated shortly after collection and dilution. Duration of fertility of spermatozoa declined from 15days to 11 days owing to the dilution of semen with the phosphate buffer. The fertilizing capacity of undiluted stored spermatozoa was severely impaired, and their duration of fertility was less than half of that of the diluted unstored ones. The damaged fertility and reduced duration of fertility of stored spermatozoa are likely to be associated with the increased number of deformed spermatozoa during storage.
社団法人日本動物学会の論文
1989-08-15