Studies on the Sawfly, Athalia rosae (Insecta, Hymenoptera, Tenthredinidae). II. Experimental Activation of Mature Unfertilized Eggs : Developmental Biology

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概要

• 論文の詳細を見る

• Mature unfertilized eggs (primary oocytes arrested at metaphase of the first meiotic division) of Athalia rosae can be explanted from ovaries and placed on a filter paper soaked with distilled water, or submerged in distilled water where they are activated and start and complete embryonic development. Eggs placed and kept in a saline solution (0.15 M NaCl) are not activated, show no signs of development and deteriorate after a few hours. However once activated in distilled water for 20min, eggs can then be placed in saline solution and development continues. This 20-min period corresponds to the time required by the arrested metaphase nucleus to proceed to telophase. Various ions as well as osmolality are involved in this egg activation process in varying capacities. Other treatments such as brief desiccation, passage through narrow capillary tubing, pricking with a glass needle or lowering pH of the solution are also found to be effective in activating the eggs to various degrees.

• 社団法人日本動物学会の論文

著者

• OISHI KUGAO

  Department of Biology, Faculty of Science, Kobe University

• Oishi Kugao

  Department Of Biology Faculty Of Science Kobe University

• Sawa Masami

  Division Of Science Of Biological Resources Graduate School Of Science And Technology Kobe Universit

• Sawa Masami

  Division Of Science Of Biological Resources Graduate School Of Science And Technology Kobe Universit

• SAWA Masami

  Division of Environmental Science, Graduate School of Science and Technology, Kobe University

関連論文

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• Studies on the sex-specific lethais of Drosophila melanogaster. : VII. Sex-specific lethals that do not affect dosage compensation
• Studies on the sex-specific lethals of Drosophila melanogaster. : VI. An autosomal, recessive, non-maternal effect female-specific lethal mutant
• Studies on the sex-specific lethals of Drosophila melanogaster. : III. A third chromosome male-specific lethal mutant
• Studies on the Sawfly, Athalia rosae (Insecta, Hymenoptera, Tenthredinidae). I. General Biology : Developmental Biology
• Studies on the Sawfly, Athalia rosae (Insecta, Hymenoptera, Tenthredinidae). III. Fertilization by Sperm Injection : Developmental Biology
• Studies on the Sawfly, Athalia rosae (Insecta, Hymenoptera, Tenthredinidae). II. Experimental Activation of Mature Unfertilized Eggs : Developmental Biology

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