The 5S RNA Binding Protein of Artemia salina Ribosomes : Identification and Immunological Comparison with That of Rat Liver : Biochemistry

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概要

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• The ribonucleoprotein complex between 5S RNA and its binding protein of Artemia salina ribosomes was released from 60S subunits with 25mM EDTA. The protein component of the complex was found to contain a single ribosomal protein which seemed to correspond to rat liver ribosomal protein L5 in two-dimensional gel electrophoresis. We therefore designated the 5S RNA binding protein of Artemia salina as Artemia L5 (AL5) when compared with rat liver L5. The molecular weight of AL5 was about 34,000 in SDS-polyacrylamide gel electrophoresis and was similar to rat liver L5. The 5S RNA-AL5 complexes were injected into rabbits intradermally, and antisera against AL5 were obtained. Cross-reactivity of the anti-AL5 with rat liver 60S proteins was examined by immunoblotting and the anti-AL5 was found to cross-react with rat liver L5. This result indicated that Artemia and rat liver L5 share immunological determinants. These results suggest that 5S RNA binding proteins are probably conserved during the evolution of eukaryotic cells and may play a significant role in ribosomes.

• 社団法人日本動物学会の論文
• 1989-04-15

著者

• Kenmochi N

  Department Of Biochemistry Niigata University School Of Medicine:(present)facilities For Comparative

• Kenmochi Naoya

  Department Of Biochemistry School Of Medicine University Of The Ryukyus

• OGATA KIKUO

  Department of Biochemistry, Niigata University School of Medicine

• Ogata Kikuo

  Department Of Biochemistry Niigata University School Of Medicine:(present)institute For Gene Express

関連論文

• The 5S RNA Binding Protein of Artemia salina Ribosomes : Identification and Immunological Comparison with That of Rat Liver : Biochemistry
• The human ribosomal protein L6 gene in a critical region for Noonan syndrome

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