A Possible Sugar Receptor Protein Found in the Labellum of the Blowfly, Phormia regina : Physiology

概要

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Previous physiological studies have suggested the existence of at least three functionally separated receptor sites in the labellar sugar receptor of the fly, called the pyranose (P site), the furanose (F site) and the third sites (T site), and that starch acts as a competitive inhibitor for the P site. I detected in this work a new candidate protein for the P site in the labellar extract by affinity electrophoresis with starch. The dissociation constant of the candidate protein-starch complex was estimated to be 0.7%, a value consistent with the electrophysiological estimate of the inhibition constant for starch on the sugar response. The stimulus sugars for the P site bound to the candidate protein in competition with starch. The dissociation constants of the candidate protein-sugar complexes were highly correlated with the electrophysiological constants defined as the sugar concentrations which give rise to half maxitnal responses. However, the stimulus sugars for the F site did not compete with starch for the candidate protein. The candidate protein was water insoluble and appeared to be located in the distal process and the cell body, but not in the axon, of the labellar chemosensory cell.
社団法人日本動物学会の論文
1988-04-15