Cyclic CMP Alters Squirrel Monkey (Saimiri sciureus) Luteal Cell Structure via Cyclic AMP-Dependent Mechanisms : Endocrinology

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Cyclic cytidine monophosphate (cCMP) is a pyrimidine nucleotide that is not as well-known as the other intracellular messengers such as cyclic adenosine monophosphate (cAMP) . The present study was undertaken to determine if cCMP functions to alter cultured luteal cell morphology and if the mechanism involves cAMP. Adult female squirrel monkeys of Guyanan origin were administered daily single i.m. injections of FSH (follicle-stimulating hormone) for 4days followed by hCG (human chorionic gonadotropin) on the fourth day. Luteal cells were aspirated from ovarian corpora lutea 64hr after hCG by laparoscopy. The cells were dispersed, washed and cultured in individual center-well petri dishes for 48hr at 37℃ in 5% CO_2 in air. The test compounds (10μM each), dbcCMP, dbcAMP, dbcGMP, dbcCMP and 6mM imidazole, or control media were added at the start of culture. At the end of the incubation period, the cells were fixed in methanol and stained with Giemsa stain. The data indicated that dbcCMP restructured the polygonal luteal cells into small narrow-shaped cells with minimal cytoplasm in a manner similar to dbeAMP. However, when imidazole, a phosphodiesterase stimulator, was also present, the cells retained the polygonal shape. Dibutyryl cGMP at the concentration tested did not affect luteal cell morphology. The results suggest that dbcCMP alters the squirrel monkey luteal cell morphology in a manner similar to dbcAMP and that the process requires intracellular cAMP.
社団法人日本動物学会の論文
1987-06-15

Cyclic CMP Alters Squirrel Monkey (Saimiri sciureus) Luteal Cell Structure via Cyclic AMP-Dependent Mechanisms : Endocrinology

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