Fluorescence Image Analysis for Quantification of Reactive Oxygen Species Derived from Monocytes Activated by Photochemical Reaction(Special Issue on Measurements and Visualization Technology of Biological Information)

概要

論文の詳細を見る
This study attempts to demonstrate that activated leukocytes are involved in vascular shut down effect(VSD)in photodynamic therapy(PDT). Hydrogen peroxide(H_2O_2), a reactive oxygen specie(ROS)that is found in monocytes, was visualized under a confocal laser scanning microscope, and ROS formation was quantified by fluorescence image analysis. The fluorescence intensity was expressed as a gray level graded from 0 to 255. Only the fluorescence derived from monocytes that had ZnCP-III incorporated and were irradiated with an HeNe laser caused increases in the fluorescence distribution over time, while no change of distribution was observed in three other conditions(only Zn CP-III added, only HeNe laser irradiation, or non-treated). The result indicates that the photochemical reaction induced by excitation of a photosensitizer, and ROS was derived from the reaction-stimulated monocytes. The activated monocytes generated ROS themselves and H_2O_2 was visualized by the DCFH fluorescence method. In conclusion, the result clearly shows that activated monocytes are involved in the VSD effect.
2002-01-01