HISTOCHEMICAL DEMONSTRATION OF ACID BETA-D-GALACTO-SIDASE ACTIVITY IN CULTURED HUMAN SKIN FIBROBLASTS AND ITS APPLICATION TO SINGLE CELL ANALYSES
スポンサーリンク
概要
- 論文の詳細を見る
In 1970, Lojda reported the improved indigogenic method for β-D-galactosidase (β-gal). This method was applied to cultured skin fibroblasts from patients with three β-gal deficient disorders and from normal subjects. Cells from normal subjects showed the formation of insoluble blue indigo dye, whereas cells from the patients remained unstained. Clear-cut differences were observed in the intensity of the β-gal activities between cells from normal subjects and from the patients. This method was also applied to cultured amniotic fluid cells, and to the judgment of a genetic complementation study in restoration of β-gal activity.
- 日本組織細胞化学会の論文
著者
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FURUYAMA Jun-ichi
Department of Genetics, Hyogo College of Medicine
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OKADA Shintaro
Department of Pediatrics, Osaka University Medical School
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YUTAKA Tohru
Department of Pediatrics, Osaka Univer-ity Hospital
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Yutaka Tohru
Department Of Pediatrics Osaka Univer-ity Hospital
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Okada Shintaro
Department Of Pediatrics Osaka Univer-ity Hospital
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Kato Tomochika
Department Of Pediatrics Osaka Univer-ity Hospital
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Okada Shintaro
Department Of Pediatrics Osaka University
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KATO TOMOCHIKA
Deftartment of Pediatrics, Osaka University Hospital
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INUI KOJI
Deftartment of Pediatrics, Osaka University Hospital
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YUTAKA TOHRU
Deftartment of Pediatrics, Osaka University Hospital
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OKADA SHINTARO
Deftartment of Pediatrics, Osaka University Hospital
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YABUUCHI HYAKUJI
Deftartment of Pediatrics, Osaka University Hospital
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CHIYO HIDEKI
Department of Genetics, Hyogo Medical College
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Furuyama Jun-ichi
Department Of Genetics Hyogo Medical College
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Furuyama Jun-ichi
Department Of Genetics Hyogo College Of Medicine
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Chiyo Hideki
Department Of Genetics Hyogo Medical College
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Yabuuchi Hyakuji
Department Of Pediatrics Osaka Univer-ity Hospital
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Yabuuchi Hyakuji
Department Of Pediatrics Osaka University Medical School
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Inui Koji
Inui Children's Clinic
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