The expression of MAPs in the developing chick optic tectum

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The relationship between the maturation of neurons and the layer formation in the developing chick optic tectum has not been studied in detail. To elucidate the maturation process of neurons in the layers during development of the optic tectum, the expression of MAPs was studied, using anti-MAP1A, MAP1B, MAP2 and neurofilament-M (NF-M) antibodies. The sections of optic tecta of the embryos, incubated for 3,5,7,11,13 or 19 days and fixed in 4% paraformaldehyde, were immuno-stained and observed under a confocal microscope. On the third day of incubation, MAP1B+/NF-M+ cells were observed in the mesencephalon differentiating to the optic tectum. On the fifth day when 2 layers became first distinguishable, neuroblasts comprising layer I and layer IL consisting of efferent fibers were MAP1B+/NF-M+. The neuroblasts in layer 1, the earliest born neuroblasts which differentiate to multipolar neurons in SGC, were also MAP1A+ /MAP2+. They are the earliest neurons. Both the efferent layer and the afferent fibers were MAP1B+/NF-M+ until the thirteenth day. On the nineteenth day and thereafter the staining level of these layers became attenuated, indicating that the efferent and afferent fibers had almost finished their growth before hatching.
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