X-Ray Microanalysis of Semi-thin Sections by High Voltage Electron Microscopy

スポンサーリンク

概要

• 論文の詳細を見る

• For the purpose of analyzing and imaging chemical components of cells and tissues at the electron microscopic level, two physical methods are available. The first method is radioautography which can demonstrate the localization of radiolabeled compounds which were incorporated into cells and tissues. The second method is X-ray microanalysis which can qualitatively analyze and quantitY the total amounts of elements present in cells and tissues. We have developed the two methodologies in combination with intermediate high voltage transmission electron microscopy (200-400 kV) and applied them to various kinds of organic and inorganic compounds present in biological materials. X-ray microanalysis is a useful method to qualify and quantify basic elements in biological specimens. We first quantified the end products of histochemical reactions such as Ag in radioautograms or Ce in phosphatase activity using semi-thin sections. The P/B ratios of both Ag and Ce increased with the increase of the accelerating voltages from 100 to 400kV. Then we analyzed various trace elements such as Zn, Ca, 5, which originally existed in cytoplasmic matrix or cell organelles of various cells, using both conventional chemical fixation and cryo-fixation followed by cryosectioning and freeze-drying, or freeze-substitution and dry-sectioning, or freeze-drying and dry-sectioning producing semi-thin sections similarly to radioautography. As the results, Zn was demonstrated in Paneth cell granules of mouse intestine and its P/B ratio showed a peak at 200 kV, Ca was found in human ligaments and rat mast cells with a maximum of P/B ratios at 350 kV, S in mouse colonic goblet cells with a maximum at 300kV. From the results, it was shown that X-ray microanalysis using semi-thin sections observed by intermediate high voltage transmission electron microscopy at 200-400 kV was very useful resulting in high P/B ratios for quantifying some trace elements in biological specimens. These methodologies should be used in microanalysis of various compounds and elements in various cells and tissues in various organs.

• 日本組織細胞化学会の論文

著者

• Nagata Tetsuji

  Dept. Anat. Cell Biol. Shinshu University School Of Medicine

• Nagata Tetsuji

  Dept. Of Anatomy And Cell Biology Shinshu University School Of Medicine:dept. Of Anatomy And Physiol

関連論文

• Intracellular Localization of the Calmodulin Antagonist
• Demonstration of Small Molecular Compound ^3H-W-7 by Means of Light Microscopic Radioautography
• X-Ray Microanalysis of Semi-thin Sections by High Voltage Electron Microscopy
• Special Radioautographology : A Novel Concept(THE SIXTH JAPAN-CHINA JOINT SEMINAR ON HISTOCHEMISTRY AND CYTOCHEMISTRY)
• P1-19 High Voltage Electron Microscopy is Useful for X-Ray Microanalysis

スポンサーリンク