Change of Glycosylation State of Brain Glycoprotein during Aging

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概要

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• Glycoproteins in the soluble fraction and in the membrane fraction of various portions of brains and spinal cords, obtained from 9-week-old rats and 29-month-old rats, were comparatively analyzed by SDS-polyacrylamide gel electrophoresis and lectin staining. The glycoprotein patterns of each brain part showed marked differences by the age of donors, indicating that the glycosylation state of the protein in the brain changes during aging. Among them, the most prominent evidence is that a glycoprotein with an apparent molecular weight of 30KDa (gp30), which react with Lens culinaris agglutinin, in the membrane preparation of spinal cords, was detected in the aged rats, but not in the young adult rats. Based on the amino acid sequence data around the glycosylation site, the gp30 was identified as P_0, which is a member of immunoglobulin superfamily and a major structural component of mammalian peripheral nerve myelin. This is the first report indicating that P_0 occurs in the spinal cord of mammals. In addition, nonglycosylated P_0 molecule could be detected in the spinal cord of young adult rats by anti-P_0 polyclonal antibody. We also determine, by immunohistochemical analysis, that the neurons in the spinal cord express P_0. Furthermore, our data show that the number of neurons expressing P_0 decreases and their sizes become smaller with age. The histochemical distribution of sialoglycoconjugates in the CA1 region in the hippocampus formation of young adult and aged rats was examined using electron microscopy in combination with two lectins, Maackia amurensis lectin, specific for Sia_α2-3Gal, and Sambucus sieboldiana agglutinin, specific for Sia_α2-6Gal. Each lectin stained the plasma membranes of pyramidal cells, indicating that the Sia_α2-3Gal and Sia_α2-6Gal groups were expressed on their plasma membranes. Additionally these lectins also bound to synapses in the stratum lacunosum-moleculare. The staining intensity of the lectins in the synapses in these layers was down-regulated in the aged rats. These results indicated that both the Sia_α2-3Gal and Sia_α2-6Gal groups were expressed on these synapses and that the expression of these sialyl linkages decreased in the aged brain.

• 日本組織細胞化学会の論文

著者

• Endo Tamao

  Department Of Glycobiology Tokyo Metropolitan Institute Of Gerontology Tokyo

• Endo Tamao

  Department Of Glycobiology Tokyo Metropolitan Institute Of Gerontology

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• Change of Glycosylation State of Brain Glycoprotein during Aging

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