QUICK-FREEZE DEEP ETCH REPLICA OBSERVATION OF THE CYTOSKELETON COMBINED WITH ANTIBODY DECORATION (<Symposium>Histo- and Cytochemistry in Tissue Culture)

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概要

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• The recently developed quick freeze, deep-etch technique (QF・DE) enables us to observe three-dimensional architecture of cytoskeletal elements at a resolution high enough to pick out macromolecular structures. The chemical nature of the cytoskeletal polymers can also be investigated by in-situ decoration with antibodies or immunocytochemistry in conjunction with the QF・DE. Here we present molecular structures of actin, and intermediate filaments, associated cytoplasms by these approaches.

• 日本組織細胞化学会の論文

著者

• Hirokawa Nobutaka

  東京大学 解剖

• Hirokawa Nobutaka

  Department Of Anatomy And Cell Biology Faculty Of Medicine

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• QUICK-FREEZE DEEP ETCH REPLICA OBSERVATION OF THE CYTOSKELETON COMBINED WITH ANTIBODY DECORATION (Histo- and Cytochemistry in Tissue Culture)

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