IMMUNOENZYMATIC LABELLING PATTERN OF DIHYDROFOLATE REDUCTASE USING ALKALINE-PHOSPHATASE/ANTI-ALKALINE PHOSPHATASE (APAAP) METHOD IN HAEMIC CELLS

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概要

• 論文の詳細を見る

• The availability of anti-human dihydrofolate reductase polyclonal antibodies, allowed us to develop an immunohistochemical method improving the tetrazolium salt method for the demonstration of dihydrofolate reductase in samples in peripheral blood and bone marrow. This enzyme, immunohistochemically demonstrated with the aikaline-phosphatase/anti-alkalinc-phosphatase (APAAP) method, progressively increased during the normal differentiation of the granulocytopoietic series while it decreased in the erythropoietic series. The lymphocytes showed various positivity patterns which related to their subpopulations. These results were in agreement with previous observations by the tetrazolium salt method. Dihydrofolate reductase may be a useful marker in the study of granulocytic and lymphocytic cell lineages.

• 日本組織細胞化学会の論文

著者

• REZZANI Rita

  Department of Biomedical Sciences and Biotechnology, University of Brescia

• RODELLA Luigi

  Department of Biomedical Sciences and Biotechnology, University of Brescia

• Rodella L

  Univ. Brescia Ita

• Rezzani R

  Univ. Brescia Brescia Ita

• Rezzani Rita

  Department Of Biomedical Sciences Anatomy Section University Of Brescia

• Rodella Luigi

  Department Of Biomedical Sciences Anatomy Section University Of Brescia

• NANO ROSANNA

  Department of Animal Biology and Center of Study for Histochemistry, C. N. R., University of Pavia

• GERZELI GIUSEPPE

  Department of Animal Biology and Center of Study for Histochemistry, C. N. R., University of Pavia

• Nano Rosanna

  Department Of Animal Biology And Center Of Study For Histochemistry C. N. R. University Of Pavia

• Gerzeli Giuseppe

  Department Of Animal Biology And Center Of Study For Histochemistry C. N. R. University Of Pavia

• RODELLA LUIGI

  Department of Animal Biology and Center of Study for Histochemistry, C. N. R., University of Pavia

• REZZANI RITA

  Department of Animal Biology and Center of Study for Histochemistry, C. N. R., University of Pavia

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• The Cytoarchitecture of the Adult Human Parabrachial Nucleus : a Nissl and Golgi Study
• IMMUNOENZYMATIC LABELLING PATTERN OF DIHYDROFOLATE REDUCTASE USING ALKALINE-PHOSPHATASE/ANTI-ALKALINE PHOSPHATASE (APAAP) METHOD IN HAEMIC CELLS
• Apoptosis induced by NK cells is modulated by the NK-active cytokines IL-2 AND IL-12

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