RELATIONSHIP BETWEEN THE STAINING PROPERTY OF MAST CELL GRANULE WITH ALCIAN BLUE-SAFRANIN O AND TOLUIDINE BLUE O, AND THE CONTENT OF MAST CELL PROTEASE I IN THE GRANULE OF RAT PERITONEAL MAST CELL

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概要

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• The relationship between the amount of rat mast cell protease I (RMCP I) in the granulcs and the stainability of granules was examined using the peritoneal mast cell of the Sprague-Dawley rat (6 weeks old). Epon sections of rat peritoneal mast cells were stained immunohistochemically for RMCP I, and observed with an electron microscope. Consecutive sequcntial light microscopic sections were stained with toluidine bluc O. Photographs of electron microscopy and light microscopy of the same mast cells were compared each other. The dark violet granules with toluidine blue O contained the largest amount of RMCP I, the purple or intermediately stained granules less amount of RMCP I, and the pink granules slight amount of RMCP I. The mast cells, which were fixed with glutaraldchyde and osmium tetroxide and stained with alcian blue-safranin, followed by epon embedding, showed various colors of granules, if roughly divided, safranin positive, intermediately stained, and alcian blue positive granules. These sections were further stained with toluidine blue O and it was found that the safranin positive, the intermediately stained and the alcien blue positive granules with alcian blue-safranin double staining corresponded to the dark violet, purple and pink granules with the toluidine blue O staining, respectively. Thus it was concluded that light microscopic observations by toluidine blue O staining and/or alcian blue-safranin O double staining which is more generally acccpted in regard to the distinction of mast cell granules than toluidine blue O staining enabled us to estimate the amount of RMCP I in the mast cell granules. This result will be useful to interpret the functional activity of mast cell populations in various physiological and pathological conditions.

• 日本組織細胞化学会の論文

著者

• KORETOU Osamu

  Department of Pathology, University of Shiga Medical Science

• Koretou Osamu

  Department Of Pathology Shiga University Of Medical Science

関連論文

• Relationship between Stainability of Granules and Rat Mast Cell Protease I in Granules of Rat Peritoneal Mast Cell
• ACID PHOSPHATASE REACTION AND ALCIAN BLUE-SAFRANIN STAINING OF RAT PERITONEAL MAST CELLS
• Acp reaction and alcian blue-safranine staining of rat peritoneal mast cells
• Histochemical staining of mast cell granule with avidin-HRP
• RELATIONSHIP BETWEEN THE STAINING PROPERTY OF MAST CELL GRANULE WITH ALCIAN BLUE-SAFRANIN O AND TOLUIDINE BLUE O, AND THE CONTENT OF MAST CELL PROTEASE I IN THE GRANULE OF RAT PERITONEAL MAST CELL

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