GLYCOCONJUGATES OF SECRETORY GRANULES IN THE MIDDLE EAR MUCOSA AS REVEALED BY POST-EMBEDDING STAINING WITH LECTIN-GOLD COMPLEXES
スポンサーリンク
概要
- 論文の詳細を見る
Granules of the secretory cells of guinea pig middle ear mucosa, including those of the eustachian tube, were studied by electron microscopic lectin-gold complex postembedding staining. Electron-lucent (EL) granules were intensely stained with wheat germ agglutinin (WGA)-gold and Helix pomatia agglutinin (HPA)-gold. Within these granules WGA-gold stained the entire electron-dense core and the surrounding filamentous structure. In contrast HPA-gold stained the filamentous structure and only the lesser electron-dense portion of the core. The electron-dense (ED) granules were uniformly labeled with WGA, but did not stain with HPA. In intermediate cells, a small number of a third type of granulc (intermediately electron-densc (ID)), which resembled ED granules but were less electron-dense and had a peripherally electron-lucent zone adjacent to the limiting membrane, showed a weak reactivity with HPA only on the limiting membrane. Thesc results suggested that one type of granule could transform into another.
- 日本組織細胞化学会の論文
著者
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Tachibana Masayoshi
Department Of Electronic And Photonic System Engineering Kochi University Of Technology
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Morioka Hiroyuki
Laboratory Of Electron Microscopy Kyoto Prefectural University Of Medicine
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Tanimura Fumiko
Department Of Otolaryngology Municipal Yamashiro Hospital
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