ORIGIN OF MYOTOME CELLS IN THE CHICK EMBRYO STUDIED BY ^3H-THYMIDINE AUTORADIOGRAPHY AND ACETYLCHOLINESTERASE HISTOCHEMISTRY
スポンサーリンク
概要
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^3H-thymidine autoradiography and acetylcholinesterase (AChE) histochemistry were performed on the same plastic sections to examine the origin of myotome cells in the chick embryo. The myotome first forms in the dorso-medial angle of the dermomyotome and elongates ventro-laterally along the inner surface of the dermomyotome with development. The myotome consists of numerous AChE-positive cells and some AChE-negative cells. Two hr after injection of ^3H-thymidine, a few AChE-negative cells but none of the AChE-positive myotome cells were labeled. Four hr after the injection, a few labeled AChE-positive and AChE-negative cells were observed throughout the myotome and thereafter, the labeling of the AChE-positive as well as AChE-negative cells increased with time. Twenty-four hr after the treatment, many labeled cells were distributed throughout the myotome, but unlabeled AChE-positive cells were also evident in the myotome. In the present study, the proliferating ability and origin of myotome cells in the chick embryo are discussed.
- 日本組織細胞化学会の論文
著者
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Miki Akinori
Department Of Anatomy Kobe University School Of Medicine
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Mizoguti Humio
Department Of Anatomy Division Ii Kobe University School Of Medicine
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