A CYTOCHEMICAL STUDY OF BENIGN AND MALIGNANT CARTILAGINOUS TUMORS
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概要
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Twelve cases of benign and malignant cartilaginous tumors were subjected to cytological and cytochemical studies using human fetal cartilage as control. They included 4 cases of chondrosarcoma, 2 cases of benign chondroblastoma, and 3 cases each of enchondroma and osteochondroma (exostosis). An electron microscope was used. The ultrastructural features and cytochemical localization of the reaction product of polysaccharide and alkaline phosphatase activity (ALPase) of the tumor cells of well-differentiated chondrosarcoma, enchondroma and osteochondroma were similar to those of the distal zones of the epiphyseal plate of the controls. These results suggested tumor cell functions may be similar to those of the controls as well as normal chondrocytes. Undifferentiated chondrosarcoma cells, however, had poorly developed organellae and a few vacuoles with deposits of periodic-acid methenamine-silver staining (PAM) in their cytoplasm. An abundance of ALPase reaction product was observed in their cytoplasmic membranes but scarcely any in their cytoplasm. These findings suggest decreased matrix synthesis (especially proteoglycans) in the cells of undifferentiated chondrosarcoma. Benign chondroblastoma cells demonstrated well-developed rough-endoplasmic reticula (r-ER) in contrast to the Golgi apparatuses, and PAM-reaction products were not seen in their cytoplasm. On the basis of the ultrastructural localization of ALPase activity, two different cell types were seen in benign chondroblastoma: fetal cartilage-like cells (type A), and peculiar cells which had abundant deposits of ALPase in the enlarged r-ER but not on the cytoplasmic membrane (type B). These findings suggested an interference in the matrix synthesis process of r-ER in type B benign chondroblastoma cells. It was concluded that the ultrastructural localization of both ALPase and polysaccharide reaction products could be useful in defining cell function and the level of cell differentiation in carti-laginous tumors.
- 日本組織細胞化学会の論文