ULTRACYTOCHEMICAL DEMONSTRATION OF OUABAIN-SENSITIVE, K^+-DEPENDENT P-NITROPHENYLPHOSPHATASE (NA^+-K^+-ATPASE) ACTIVITY IN THE RAT HIPPOCAMPAL FORMATION

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概要

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• The localization of ouabain-sensitive, K^+-dependent p-nitrophenylphos-phatase )K^+-NPPase) activity, a part of the reaction of the Na^+-K^+-ATPase complex, was ultracytochemically investigated in the rat hippocampal formation called substantia gelatinosa cerebri. Microslicer sections of the hippocampal formation fixed with a mixture of 0.25% glutaraldehyde and 1% para-formaldehyde for 30 min were incubated in the medium for K^+-NPPase. In the light microscopic observations, the most abundant reaction products were present in the stratum moleculare of the hippocampal formation. Electron microscopically, K^+-NPPase activity was demonstrated on the axolemma, neurofilamentous structures in the axoplasm and synaptic plasma membrane. These activities were distinctly decreased with addition of 10 mMouabain or substitution of Na^+ for K^+. Additionally, ouabain-insensitive, K^+-independent p-NPPase activity was observed on the mitochondria, nuclear envelope and endoplasmic reticulum. Neurotubules did not show any K^+-NPPase activity.

• 日本組織細胞化学会の論文

著者

• Nasu Fumio

  Electron Microscopy Research Laboratory Shimane Medical University

関連論文

• Ultracytochemical localization of ouabain-sensitive, K^+-dependent p-nitrophenylphosphatase activity in the rat hippocampus.
• ULTRACYTOCHEMICAL DEMONSTRATION OF OUABAIN-SENSITIVE, K^+-DEPENDENT P-NITROPHENYLPHOSPHATASE (NA^+-K^+-ATPASE) ACTIVITY IN THE RAT HIPPOCAMPAL FORMATION

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