DISTRIBUTION OF GLYCOCONJUGATES IN NORMAL RAT KIDNEY DETERMINED BY USING HORSERADISH PEROXIDASE-LABELED LECTINS
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概要
- 論文の詳細を見る
Lectins have been widely used as probes of the structure and organization of cell membrane saccharide-containing components. To determine the distribution of glycoconjugates in different areas of the rat kidney, seven different horseradish peroxidase-labeled lectins were used in the present study. The distribution of binding sites of several lectins in the proximal tubules was of special interest. The brush borders of the proximal tubules reacted differently to labeled lectins at each segment. This indicates that differences in carbohydrate composition of the brush border exist in the various segments. The ascending limb of Henle's loop (ALH) had strong binding for peanut agglutinin (PNA) and soybean agglutinin (SBA). Dolichos biflorus agglutinin (DBA) appears to be a good marker for distinguishing ALH from the distal convoluted tubule. DBA, SBA and PNA also serve as markers for the collecting tubules. In combination with various lectins, findings concerning heterogeneity in the distribution of the renal glycoconjugates were elucidated. In addition, the fine structural localization of glycoconjugates which react with some of the lectins was observed electron microscopically.
- 日本組織細胞化学会の論文
著者
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Hamada Hiroshi
Department Of Urology And Department Of Anatomy Kagoshima University School Of Medicine
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Hamada Hiroshi
Department Of Anesthesia Onomichi General Hospital
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