HISTOCHEMICAL OBSERVATIONS OF GLYCOGEN SYNTHETASE ACTIVITY USING GDPG AND TDPG AS SUBSTRATE IN RAT SKELETAL MUSCLE FIBERS

概要

論文の詳細を見る
Uridine diphosphoglucose (UDPG) is conventionally used as a substrate for the histochemical demonstration of glycogen synthetase activity in mammalian tissues. In the present study, glycosyl nucleotides other than UDPG, guanosine diphosphoglucose (GDPG) and thymidine diphosphoglucose (TDPG), acted as the substrate to demonstrate the glycogen synthetase activity in rat skeletal muscle fibers. The histochemical reaction using GDPG and TDPG was very similar in its location but not in its intensity to the UDPG one. Furthermore, the different size and shape of the new glycogen particles synthesized from GDPG and TDPG by the enzyme activity were observed according to the type of skeletal muscle fibers under the electron microscope. The results presented here indicate that GDPG and TDPG as well as UDPG act as glucosyl donors for new glycogen synthesis through the glycogen synthetase activities in rat tissues, and characteristic glycogen particles can be observed under light and electron microscopic conditions.
日本組織細胞化学会の論文