CYTOCHEMICAL STUDY OF TETRAZOLIUM REDUCTION BY ISOLATED VICIA CHLOROPLASTS UNDER ILLUMINATION
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概要
- 論文の詳細を見る
A concurrent microchemical and topochemical study of the tetrazolium reduction by isolated chloroplasts of Vicia faba under illumination was carried out. The microchemical analysis with INT revealed that the photosystem responsible for the reduction was the PS-II, because the addition of DCMU, DCPA, MCC, CIPC or CAT to the reaction medium at low concentrations markedly suppressed the formazan production. The topochemical analysis with BSPT, combined with the densitometric one, revealed that the electron transfer activity mediated by the PS-II was localized in the thylakoid membrane proper, and extended to the surface of the loculus. This result is in harmony with the spatial localization of the PS-II complex particles on the EF face and the ES of the thylakoid membrane as demonstrated by the freeze fracture technique.
- 日本組織細胞化学会の論文
著者
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Hiraoka Toshisuke
Department Of Biology School Of Med. Shiga University Of Medical Science
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IMAIZUMI MACHIKO
Department of Biology, School of Medicine, Shiga University of Medical Science
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Imaizumi Machiko
Department Of Biology School Of Medicine Shiga University Of Medical Science
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Imaizumi Machiko
Department Of Biology School Of Med. Shiga University Of Medical Science
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