HISTOCHEMICAL OBSERVATIONS ON POLYGLUCOSES SYNTHESIZED BY ENZYME ACTIVITY IN CARTILAGE CELLS

概要

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Polyglucose particles artificially synthesized under histochemical conditions from glucose 1-phosphate by phosphorylase and branching glycosyltransferase in various cartilage cells were observed by light and electron microscopy. In chick embryos, the polyglucoses were stained red-violet or violet-blue with iodine, and the polyglucose particles were observed electron microscopically to be spherical branching bodies with an average diameter of 369±61.62 Å. In the adult chicken, they were stained reddish-violet with iodine and formed similar particles with an average diameter of 357±65.96Å. In the human fetus, the polyglucoses were stained red-violet with iodine and the particles were observed as larger bodies with an average diameter of 529±94.98 Å. In the human adult, they were stained red-brown with iodine and electron microscopic findings revealed particles which were relatively uniform and had an average diameter of 373±56.05 Å. Polyglucoses synthesized in tumorous cartilage cells of human chondrosarcoma were stained red-violet with iodine and the particles were larger and more irregular in size. The average diameter was 540±169.76 Å. These particles also appeared in the intranuclear regions, suggesting enzyme deviation as a result of carcinogenesis.
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