HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL STUDY OF LIPASE IN MOUSE PANCREAS

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概要

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• To examine the intracellular localization of the pancreatic lipase in the mouse pancreas, both histochemical and immunohistochemical stainings were applied on the ultra-thin frozen sections. Furthermore, in order to study the mutual relationship of the distribution between lipase and α-amylase as well as α-amylase and chymotrypsinogen A within a zymogen granule, immunohistochemical double staining was performed. The positive reaction for lipase by the histochemical staining were observed in the peripheral zone of the zymogen granules, as belt-like electron opaque deposits of 20 Å to 40 Å in width. Especially, in the isolated zymogen granules, the positive reaction sites were clearly recognized inside the limiting membrane of the zymogen granule as electron dense deposits of 30 Å to 40 Å in diameter. By the immunohistochemical study, lipase was localized along the limiting membrane of the zymogen granule, while, α-amylase and chymotrypsinogen A were distributed diffusely in a zymogen granule. Moreover, when the isolated zymogen granules were treated with alkaline solution, large amounts of lipase remained in the membrane fraction, but most α-amylase activity (more than 95%) was extracted into the soluble fraction. This fact suggests that lipase is more closely associated with the limiting membrane of the zymogen granule, than α-amylase. From these results, the localizing pattern of the pancreatic lipase was found to be different from those of α-amylase, chymotrypsinogen A and trypsinogen; the former bound loosely to the inside of the limiting membrane of the zymogen granule, while the latter enzymes were localized diffusely within a zymogen granule.

• 日本組織細胞化学会の論文

著者

• Ono Tetsuro

  Department Of Anatomy Kitasato University School Of Medicine

• Ono Tetsuro

  Department Of Anatomy School Of Medicine Kitasato University

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• HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL STUDY OF LIPASE IN MOUSE PANCREAS

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