FINE STRUCTURAL DEMONSTRATION OF GLUCOSE IN MOUSE LIVER AND KIDNEY WITH A NEW TECHNIQUE
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概要
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Since hydrogen peroxide is produced from glucose oxidized by glucose oxidase, the histochemical localization of glucose can be demonstrated at the electron microscopic level by capturing this hydrogen peroxide. Iron ions show good results as capture reagents. Cytochemical localization of glucose in mice liver and kidney are observed as fine precipitates in the periphery of glycogen area, rough endoplasmic reticulum and others. These evidences agree well with the localization of glucose-6-phosphatase activity. In the kidney of mice, a lot of deposits of reaction products can be seen in the basal infolding membrane of epithelial cells of convoluted tubules and less deposits in the microvilli, and others. These findings are thought to be in good agreement with the phosphorylation-dephosphorylation theory for the reabsorption of glucose.
- 日本組織細胞化学会の論文