DYNAMIC MICROSPECTROPHOTOMETRY OF FRESH CELLS
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概要
- 論文の詳細を見る
A relative content of rhodopsin in outer segment of fresh frozen section of the retina was measured by means of microspectrophotometry with a wavelength at 500 nm. A typical process in bleaching and regeneration of rhodopsin could be recorded in situ. Microspectrophotometry was further adapted to study activities of lactate and succinate NBT-reductase system in a fresh frozen section. Courses of reduction of NBT during histochemical procedures could be recorded in situ. This method bridges the gap between histochemistry and enzymology in the fresh cells, especially regarding fundamental energy-linked cell reactions.
- 日本組織細胞化学会の論文
著者
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Mizuno Katsuyoshi
Department Of Ophthalmology Nagoya City University Medical School
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Miyata Mikio
Department Of Ophthalmology Nagoya City University Medical School
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Shinohara Yoshitaka
Department Of Ophthalmology Nagoya City University Medical School
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YAMAZAKI IKUO
Department of Ophthalmology, Nagoya City University Medical School
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Yamazaki Ikuo
Department Of Ophthalmology Nagoya City University Medical School
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MIYATA Mikio
Department of Ophthalmology, Nagoya City University Medical School
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