ELECTRON MICROSCOPIC DEMONSTRATION OF LACTATE DEHYDROGENASE AND NADH DEHYDROGENASE (DIAPHORASE) IN PHAGOSOMES (PLENARY SESSION ON RECENT ADVANCES IN ELECTRON MICROSCOPIC CYTOCHEMISTRY)

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The electron microscopic cytochemical localizations of lactate dehydrogenase (LDH) and NADH dehydrogenase (diaphorase, NADH-D) were studied in osteoclasts of developing chick bone and of mandibular condyle of newborn mice. These enzymes were also studied in trigeminal ganglion neurons of newborn mice. Both of these enzymes were observed in some mitochondria in all tissues, suggesting involvement in aerobic metabolism; their association with phagocytic vacuoles suggests involvement in anaerobic glycolysis, also. Lactic acid produced in lysosomes and phagosomes could enhance the activities of acid hydrolases in the digestion of extracellular and intracellular substances. An NADH-D isoenzyme with a relationship to anaerobic metabolism, like the LDH muscle isoenzyme, may be involved.
日本組織細胞化学会の論文