THE IMMUNO-MICROFLUORIMETRIC METHODS AND ITS PRACTICAL APPLICATIONS : ON THE IMMUNOFLUORESCENCE STUDY OF THE MOUSE LENS CRYSTALLINS (Symposium on the Chinical Application of Histochemistry and Cytochemistry)
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概要
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In this study, I used an Olympus single beam microfluorimeter which was modified from the multi-purpose single beam microspectrophotometer according to my specifications. This instrument was used to the sample the accuracy of localization of mouse lens crystallins by direct and indirect immunofluorescence methods. Some of the problems involved in microfluorimetry include: the necessity of the constant intensity of the exciting light, the choice of the filter system and the spot exciting method. From a practical point of view it was concluded that the indirect method of immunofluorescence proivdes a more accurate localization of each lens crystal-lin than the direct method.
- 日本組織細胞化学会の論文
著者
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Ikeda Akira
Department Of Anatomy School Of Medicine Hiroshima University:(present Address) Department Of Anatom
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Ikeda Akira
Department Of 1st Anatomy School Of Medicine Hiroshima University
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