TETRAZOTIZED BENZIDINE AS DIAZONIUM SALT USED IN AN AZO-DYE METHOD FOR THE HISTOCHEMICAL DEMONSTRATION OF ALKALINE PHOSPHATASE ACTIVITY

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An attempt was made for a histochemical demonstration of acid and alkaline phosphatases in the rat kidney and intestine, employing naphthyl phosphate as a substrate and tetrazotized benzidine as a new diazonium salt for coupling. By light microscopy, the azo dye deposit which was formed showed the site of these two kinds of enzymes to be reddish brown in color. Electron microscopy indicated that the enzyme reaction product yielded by alkaline phosphatases consists of electron opaque, fine grains on the duodenal epithelium of the rat. However, the reaction product by acid phosphatases tested in the rat kidney was so low in electron opacify that the lysosomes in the tissue did not show any appreciable increase in contrast. These results show a usefulness of tetrazotized benzidine in the azo-coupling technique for light- and electron-microscopic localization of at least alkaline phosphatases.
日本組織細胞化学会の論文