LOCALIZATION OF DOPAMINE-β-HYDROXYLASE IN BOVINE ADRENAL GLAND AND RAT SCIATIC NERVES BY THE IMPROVED ENZYME-IMMUNOCYTOCHEMICAL AND ENZYME-IMMUNOFLUO-RESCENT METHODS

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概要

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• An improved immunocytochemical method was developed in order to reduce non-specific staining, and it was compared with an improved immunofluorescent method. In these improved methods, antiserum to rabbit immunoglobulin (IgG) was purified by affinity chromatography, and then conjugated with peroxidase by the Nakane's method for the purpose of immunocytochemical method and, also with FITC for immunofluorescent method. By these procedures, non-specific staining became very low comparing with the method using commercially available fluorescein isothiocyanate (FITC) conjugate, and specificity in the staining was increased. Another new trial was made for the immunofluorescent method. The BG12 exciting filter had been frequently used for fluorescence microscopy, but the use of a Leitz KP490 filter and a BG38 filter instead of the BG12 was found to be effective to reduce non-specific fluorescence and to increase the fluorescence of specific staining.

• 日本組織細胞化学会の論文

著者

• Nagatsu Ikuko

  Laboratory Of Molecular Biology Institute For Medical Sciences School Of Medicine Fujita-gakuen Univ

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• LOCALIZATION OF DOPAMINE-β-HYDROXYLASE IN BOVINE ADRENAL GLAND AND RAT SCIATIC NERVES BY THE IMPROVED ENZYME-IMMUNOCYTOCHEMICAL AND ENZYME-IMMUNOFLUO-RESCENT METHODS

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