COLD FEULGEN HYDROLYSIS : ITS EFFECT ON DISPLACEMENT OF TRITIATED THYMIDINE

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概要

• 論文の詳細を見る

• The effect of cold 5 N HC1 (25℃±3) and hot (60℃) 1 N HC1 Feulgen hydrolysis procedures were compared for retention of tritiated thymidine as well as dye binding. Phytohemagglutinin (PHA) stimulated human lymphocytes were cultured using conventional procedures, pulse labeled with one microcurie ^3HTdR per ml cell suspension, stained with Schiff using conventional (1 N HC1-60℃) and cold (5 N HC1-25℃) procedures. The developed autoradiographs were then analyzed as to mean grain count over-all labeling index andqualitative colorometric properties. The results indicate that the plateaus for maximal retention of ^3HTdR as well as binding of Schiff reagent in phytohemagglutin (PHA) stimulated lymphocytes is best achieved using cold hydrolysis times as recommended in this study.

• 日本組織細胞化学会の論文

著者

• Elias Julius

  Basic Health Sciences Health Sciences Center State University Of New York At Stony Brook

• CONKLING KATHERINE

  Medical Research Center, Brookhaven National Laboratory

• MAKAR MICHAEL

  Medical Research Center, Brookhaven National Laboratory

• Conkling Katherine

  Medical Research Center Brookhaven National Laboratory

• Makar Michael

  Medical Research Center Brookhaven National Laboratory

関連論文

• COLD FEULGEN HYDROLYSIS : ITS EFFECT ON DISPLACEMENT OF TRITIATED THYMIDINE

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