HISTOCHEMISTRY OF PHOSPHATASE ACTIVITIES IN THE TASTE BUD OF RATS AND RABBITS(Symposium on Histochemistry of the Sensory Organs)

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Phosphatase activities are observed in the gustatory epithelium of rats and rabbits. Among nucleoside phosphatases, ATPase activity is present on taste hairs, the membrane of taste bud cells, subgemmal nerve fibers and capillary walls. The ATPase activity on the membrane of taste bud cells can be differentiated from that of the other sites using PCMB (2.5×10^<-5> M). With an electron microscope, the reaction product is present between intragemmal nerve fibers and dark cells, whereas it is almost absent on the site in contact with the light cell. An intense alkaline phosphatase activity is present on the superficial layers of the epithelium covering the gutter of foliate and circumvallate papillae. The activity disappears and reappears according to the decrease and increase of taste buds after denervation and reinnervatiorn of the glossopharyngeal nerve. Alk. Pase is not localized on the fungiform papilla in control, but is clearly demonstrated after fasting and the suppression of salivation. The results suggest that alk. Pase may be secreted from the taste bud of fungiform papillae as well as from those of circumvallate and foliate papillae. The taste bud shows an intense activity for acid Pase. The reaction product is localized in the supranuclear region of the cell.
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