OPTIMAL FEULGEN HYDROLYSIS FOR MICROSPECTROPHOTOMETRY

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概要

• 論文の詳細を見る

• For quantitative determination of Feulgem-DNA in mouse lymphocytes three hydrolysis methods were examined. With 5 N HCl hydrolysis at 25℃, there was found a long plateau of optimum hydrolysis over 30 minutes or more, while the period of optimum hydrolysis in the conventional hydrolysis at 60℃ in 1 N HCl was a few minutes, and that in 60℃ 5 N HCl was a few seconds. Proportionality of DNA content between spermatids and lymphocytes was well preserved over the long hydrolysis time in 25℃ 5 N HCl hydrolysis. Therefore, it was concluded that 25℃, 5 N HCl hydrolysis was preferable to the conventional Feulgen hydrolysis performed at 60℃ with 1 N HCl.

• 日本組織細胞化学会の論文

著者

• Nomura Akito

  Ist Department Of Anatomy Yamaguchi University School Of Medicine

• Tomonaga Susumu

  Ist Department Of Anatomy Yamaguchi University School Of Medicine

• AWAYA KAZUHIKO

  Ist Department of Anatomy, Yamaguchi University School of Medicine

• Awaya Kazuhiko

  Ist Department Of Anatomy Yamaguchi University School Of Medicine

関連論文

• OPTIMAL FEULGEN HYDROLYSIS FOR MICROSPECTROPHOTOMETRY

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