HISTOCHEMICAL DEMONSTRATION OF URIDINE DIPHOSPHATE GLUCOSE-GLYCOGEN GLUCOSYLTRANSFERASE AND PHOSPHORYLASE IN RABBIT BLOOD AND BONE MARROW CELLS ON SMEARS AND THEIR DISTRIBUTION.
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概要
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The uridine diphosphate glucose-glycogen glucosyltransferase and phosphorylase activities in blood and bone marrow cells on smears were histochemically demonstrated by use of the improved techniques of Takeuchi's methods. To prevent destruction of cell structure and to keep the enzyme activity, the unfixed smears were immersed in the substrate mixture containing polyvinyl pyrrolidon of molecular weight 30,000 at 37℃ for 50 minutes in the glucosyltransferase demonstration and for 30 minutes in the phosphorylase technique. The substrate mixture was also improved by the fundamental experiments. Differentiation between native polysaccharide and the reaction products was performed by improved techniques. The glucosyltransferase activity was demonstrated intensely in pseudoeosinophils (Neutrophils), moderately in eosinophils and weakly in thrombocytes, lymphocytes and monocytes in the peripheral blood. It was also demonstrated in these cells in the bone marrow, and it gradually decreased in the immature cells. Megakaryocytes contained this enzyme more richly. The phosphorylase activity was more intensely demonstrated in the blood and bone marrow cells in the same series mentioned above. They were also the same cells which may contain glycogen. The contents of these enzymes and glycogen in the cells of the same series were not always balanced, but the pseudoeosinophils were richer for glycogen metabolism. The reaction products for enzymes appeared in the cell matrices among specific granules in the cytoplasm of granulocytes. No reaction for the enzyme activities occurred in the cells of erythrocytic series in the normal rabbits. The index of cell number for positive reaction was counted for the series of white cells in the peripheral blood. It was greatest in pseudoeosinophils while it was about half in lymphocytes.
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