SIMULTANEOUS LOCALIZATION OF NEUROPEPTIDES (SUBSTANCE P AND VASOACTIVE INTESTINAL POLYPEPTIDE) WITH ACETYLCHOLINE-LIKE CATION AT ELECTRON MICROSCOPIC LEVEL

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概要

• 論文の詳細を見る

• A method was devised for the simultaneous localization of neuropeptides and acetylcholine (ACh)-like cation at ultrastructural level in a paraformaldehyde fixed nervous tissue (myenteric plexus). In order to minimize the loss of tissual ACh, no membrane permeabilization was made before the immunocytochemical reaction. Conventional pre-embedding immuunoperoxidase reaction for neuropeptides detection [substance P and vasoactive intestinal polypeptide (VIP)] was followed by cytochemical tonic fixation of ACh-like cation. All the substance P- or VIP-immunoreactive nerve endings possessed punctiform precipitates of ACh-like cations situated in the small clear synaptic vesicles (30-50 nm). In the vicinity of substance P- and VIP-immunoreactive nerve terminals, some other nerve terminals containing ACh-like cations did not contain any peptide immunoreactivity.

• 日本組織細胞化学会の論文

著者

• Tsuji Shigeru

  Dipartment De Cytologie Institut Des Neurosciences Universiti P. Et M. Curie

• Anglade P

  Univ. Pierre Et Marie Curie Paris Fra

• ANGLADE PHILIPPE

  National Institute for Physiological Sciences

• Anglade Philippe

  National Inst. Physiol. Sci.

関連論文

• SIMULTANEOUS LOCALIZATION OF NEUROPEPTIDES (SUBSTANCE P AND VASOACTIVE INTESTINAL POLYPEPTIDE) WITH ACETYLCHOLINE-LIKE CATION AT ELECTRON MICROSCOPIC LEVEL
• Simultaneous localization of some neuropeptides with acetylcholine-like cation at electron microscopic level

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