Intracellular Localization and Transcriptional Activation by the Human Glucocorticoid Receptor-Green Fluorescent Protein (GFP) Fusion Proteins (Fluorescent Labeling Method)

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概要

• 論文の詳細を見る

• The initial event during cellular response to glucocorticoid hormone is rapid nuclear transfer of the cytoplasmic glucocorticoid receptor (GR). In order to monitor this process in a single living cell, we employed green fluorescent protein (GFP)-tagged GR to visuallize intracelluar localization and trafficking of the receptor protein in response to ligand. Making a series of deletion GR mutants labeled with the improved GFP mutant, we mapped the intracellular localization signals on the GR protein. In addition, we provide evidence that screening of GR ligands is possible within a single cell.

• 日本組織細胞化学会の論文

著者

• UMESONO Kazuhiko

  Department of Genetics and Molecular Biology, Institute for Virus Research, Kyoto University

• Ogawa Hidesato

  Department Of Anatomy And Neurobiology Kyoto Prefectural University Of Medicine

• Ogawa Hidesato

  School of Biological Sciences, Nara Institute for Science and Technology

• Umesono K

  Kyoto Univ. Kyoto Jpn

• Umesono Kazuhiko

  Department Of Genetics And Molecular Biology Institute For Virus Research Kyoto University

• Umesono Kazuhiko

  Department of Biochemistry, Institute for Virus Research, Kyoto University

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• Intracellular Localization and Transcriptional Activation by the Human Glucocorticoid Receptor-Green Fluorescent Protein (GFP) Fusion Proteins (Fluorescent Labeling Method)

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