Retinoblastoma Susceptibility Gene Product Retrieval in Formalin-fixed, Paraffin-embedded Tissue : A Heating Method for Enhancing Immunohistochemical Staining
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概要
- 論文の詳細を見る
We examined immunohistochemical methods to visualize the retinoblastoma susceptibility gene product (RB) in formalin-fixed, paraffin-embedded sections of the U2-OS and Saos-2 human osteosarcoma cell lines. Immunocytochemical studies showed clear staining with an anti-RB antibody in 88% of the smeared U2-OS cells and in none of the smeared Saos-2 cells. Antigen retrieval methods evaluated included microwave-oven and autoclave pretreatment of paraffin-embedded sections immersed in Dulbecco's phosphate buffered saline (pH7.4) prior to incubation with the primary antibody. Strong and discrete staining of U2-OS cell nuclei for RB was seen after autoclave treatment, stronger than that seen following microwave-oven heating. Moreover, neither microwave-oven nor autoclave pretreatment produced positive staining in the nuclei of Saos-2 cells. Therefore, tissues previously considered unsuitable for immunohistochemical analysis of the RB can be studied after autoclave treatment.
- 日本組織細胞化学会の論文
著者
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Hirasawa Yasusuke
Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine
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TSUJI YOSHIRO
Department of Pediatrics, School of Medicine, Nagasaki University
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Serra Massimo
Department Of Research Oncology Rizzoli Orthopaedic Institute
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Tsuji Yoshiro
Departments Of Orthopaedic Surgery And Pathology Kyoto Prefectural University Of Medicine Department
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Tsuji Yoshirou
Department Of Pathology Kyoto Prefectural University Of Medicine
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Hirasawa Y
Kyoto Prefectural Univ. Medicine Kyoto Jpn
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Hirasawa Yasusuke
Department Of Orthopaedic Surgery Kyoto Prefectural University Of Medicine
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Hirasawa Yasusuke
Department Of Orthopaedic Surgery
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Hirasawa Yasusuke
Departrnent Of Orthopaedic Surgery Kyoto Prefectural University Of Medicine
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Kusuzaki Katsuyuki
Departments of Orthopaedic Surgery and Pathology, Kyoto Prefectural University of Medicine, Departme
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Hirasawa Yutaka
Joint Research Clinical Laboratory Fujita Health University Hospital
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Baldini Nicola
Department Of Research Oncology Rizzoli Orthopaedic Institute
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Campanacci Mario
Department of Research Oncology, Rizzoli Orthopaedic Institute
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Kusuzaki Katsuyuki
Department Of Orthopaedic Surgery Kyoto Prefectural University Of Medicine
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Tsuji Yoshiro
Department Of Cell Physiology Atomic Disease Institute Nagasaki University School Of Medicine
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Campanacci Mario
Department Of Research Oncology Rizzoli Orthopaedic Institute
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