Application of Cationic Colloidal Gold Staining for the Demonstration of Anionic Sites

概要

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Positively charged colloidal gold (cationic col-loidal gold) stains negatively charged areas (anionic sites) on histological sections. Specimens embedded in hydrophilic resinsare especially suitable for the precise localiza-tion of anionic sites with cationic gold inpost-embedding method. Enzyme digestionon the sections embedded in hydrophilicresins before the staining with cationic goldenabled us to identify the molecules such assialic acid, chondroitin sulfate, and heparansulfate which cause anionic charge. Studiesusing cationic colloidal gold have beenpublished from various fields of biology andmedicine. In the kidney, glomerular base-ment membranes and glycocalyx of epithelialcells show anionic sites and these negativecharges are thought to play important rolesin the selective permeability in theglomerulus. Distributions of anionic sites inthe glomerular basement membranes andepithelial cells are altered in diseases and inexperimentally induced nephropathy. Mylaboratory has studied the distribution and en-zyme susceptibility of anionic sites in humansweat glands, epidermis, and skin tumors.Cationic gold stains eccrine and apocrinesweat glands in a different manner. Thiswould be helpful for an accurate diagnosis ofsweat gland tumors. Data so far obtainedsuggest cationic colloidal gold is a useful toolto study structures and functions of cells andtissues with histochemical methods.
日本組織細胞化学会の論文