タンパク質の加齢変化(<特集>老化とアフェレシス)

概要

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The increase in oxidized forms of proteins including carbonyls. methionine sulfoxide and cross-links has been reported by many groups in addition to the accumulation of heat-labile and less-active enzymes in aged animal tissues. Since oxidized forms of proteins are thermodynamically unstable, the partially unfolded proteins are assumed to be more susceptible to irregular proteolysis in aged animal tissues. Here we report a decrease in the relative abundance of calmodulin, UCH-L1 and nm23-M1 in aged mouse hippocampus detected by two-dimensional gel electrophoresis and quantitative image analysis. To investigate the involvement of oxidation in the down-regulation of these proteins, the level of methionine sulfoxide was analyzed by high-throughput MALDI-TOF mass spectrometry with post-source decay fragmentation. As a result of proteomic analysis, increased methionine oxidation was detected in those proteins in aged mouse hippocampus. Our observation suggests that the oxidation of calmodulin. UCH-L1 and nm23-M1 might be involved in the down-regulation of these proteins in aged mouse hippocampus.
日本アフェレシス学会の論文
2004-05-31