化学発癌剤による腎臓細胞の組織培養内変異 : 第1報 4-nitroquinoline-l-oxideによるラット腎細胞の変異

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Rat kidney cells were transformed in culture by one time of treatment with 3.3x10^<-6>M of 4-nitroquinoline-l-oxide(4NQO) at 37℃ for 30 min. The cells were derived from 17-day-old female rats of the JAR-2 line and treated after 3 days of primary culture. In 56 days after the treatment, a colony of transformed cells was detected in the culture, followed by the formation of other two colonies within two weeks. The transformed cells showed an epithelial appearance and grew vigorously, piling up on each other. In the control cultures, however, no rapid cell growth was detected. The 4NQO-transformed cells were found, on analysis of chromosomes 160 days after the treatment, to have the modes of hypertriploid number, i.e. 67 chromosomes for one cell line and 66 for another. On backtransplantation into hamster pouches 102 days after the 4 NQO-treatment, the transformed cells produced small nodules within 2 weeks which regressed in 4 weeks. The cells were inoculated into rats subcutaneously as well as intraperitoneally 112 days after the treatment. The results are now under observation.
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