ヒト前立腺の組織培養 : 前立腺肥大症および前立腺癌由来細胞の継代培養時における性ホルモンの影響
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A total of 74 primary cell cultures were made on the tissue specimens collected by (1) open surgery, (2) TUR and (3) biopsy from 48 patients with benign prostatic hyperplasia (BPH) and 6 with carcinoma of the prostate: and 11 subcultures of cells from the patients with benign prostatic hyperplasia (8 of cells from the specimens collected by open surgery, 1 by TUR, and 2 by biopsy) and 3 of cells from the patients with carcinoma of the prostate (all by biopsy) were successfully made. These subcultures were all prepared by explant culture. A few sex hormons (testosterone, estrone, 5α-dihydrotestosterone, diethylstilbestrol diphosphate) at various concentrations (0.1, 1.0 and 10.0 μg/ml) were allowed to act on these successfully subcultured cells in order to study their effects on the culture cells from benign prostatic hyperplasia and carcinomas of the prostate in terms of (1) cell count, (2) DNA synthesis by the cells with 3^H-thymidine (by the cover slip method), and (3) morphologic changes (by light -microscopic or electron-micrqscopic observation). A) The protease extracted from bacillus polymixa proved better in cell dispersion in the primary cell culture by suspension culture than trypsin; and it gave the highest yield of viable cells when cultured at 1000 PU/ml and at 37℃ for 90 minutes. B) The doubling time of the cells from BPH was shorter than that of the cells from the carcinomas of the prostate, that is, the former being about 10-12 hours, and the latter about 26 hours. C) Both fibroblastic cells and epithel-like cells were found in the culture cells from the carcinomas of the prostate as well as in those from BPH; however, there was no marked morphologic difference between the culture cells from the two origins. D) Effects of sex hormones on culture cells. a) Testosterone, at a concentration of 1.0 μg/ml, accelerated the growth of the cells from the carcinomas of the prostate and also increased DNA synthesis by the cells. The same hormone, at a concentration of 1.0μg/ml, inhibited the growth of the cells from both of the two origins and also suppressed DNA synthesis by them. These growth inhibition and DNA synthesis suppression appear attributable to the "toxic effect" of the agent.
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