^3H-nucleosides による in vitro autoradiography の基礎的研究と膀胱腫瘍への臨床的応用
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1. Physical and chemical factors which affect in vitro incorporation of tritiated thymidine were evaluated by using autoradiography of the Yoshida sarcoma and human bladder tumors. An adequate labeling was achieved by the following method: Incubating in Hanks' balanced salt solution or normal saline containing 10 microcuries per milliliter of high specific activity ^3H-thymidine (more than 10 curies per millimole) for one hour at 37℃, dipping with NTB-3 liquid emulsion, exposing for 7 to 14days. 2. The autoradiographs of 62 human epithelial bladder tumors and 36 normal urothelia were reviewed and correlated with the clinical and histological findings. Patients were followed up for two years. a) There was a correlation between the ^3H-thymidine incorporation and the histological grading. Mean L. I. (Labeling Index) of grade I, II, III and IV were 3.9, 5.8,1 7.7 and 16.0 respectively. Some cases, however, showed extraordinarily high L. I. with low grade. b) There was a correlation between the ^3H-thymidine incorporation and the histological staging. Mean L. I. of stage A, B and C were 4.3, 13.0 and 19.1 respectively. c) There was no difference between the L.1. of ^3H-cytidine of the tumor cells and the urothelial cells. Most L. I. were more than 80. d) Normal urothelial cells showed less than 5 of ^3H-thymidine L. I. in most cases, but few were found to have very high L. I. e) Patients with tumors of high ^3H-thymidine labeling showed poorer prognosis than those of low ^3H-thymidine labeling. f) The ^3H-thymidine autoradiography is an effective method for establishing therapy plans and evaluating the prognosis of the patients with bladder tumors.
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