人膀胱腫瘍の培養及び抗癌剤感受性試験に関する研究

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For the selection of optimal anti-cancer drugs, the sensitivity test using egg culture has been attempted. The use of chorioallantoic membrane of the chick embryo for heterogenous tumor growth dates back to the classic studies of Murphy. (1912) Since then this method of egg culture has been modified by various investigators. Fertile white leghorn eggs incubated at 37℃ from nine to thirteen days were used in the present study. Human bladder tumor was surgically obtained, which was cut into fragments approximately 1 mm^3 in size. A single fragment of tumor tissue was placed on the major blood vessel branches in the chorioallantoics. Two days after tumor plantation, anti-cancer drugs such as 5-Fluorouracil (5 FU), Mitomycin C (MMC) and Carbazilpuinone (CQ) were injected into the yolk sack, and two days later the tumor was removed and examined with a light microscope and electron microscope. The following results were obtained. Bladder tumors from 48 patients were implanted and examined, and their successful plantation rate was 83.4%. Sensitivity tests of anti-cancer drugs for 30 patients were performed. Subsequently the drug was chosen according to this result, 28 patients were successfully treated during the past 18 months.
社団法人日本泌尿器科学会の論文