腎性高血圧症についての研究 : 第6報腎 Angiotensinase についての検討

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Inhibitory activity of saline extract of rabbit kidney on angiotensin II (AT II) was studied. The renal extract was prepared as follows; A kidney was removed from a normal rabbit and was separated into two portions, medulla and cortex. Each was homogenized in cold saline, centrifuged at high speed and the supernatant was diluted to the volume of thirty times of the original tissue weight with saline. Four milliliters of the extract was added with 1 ml. of AT II solution containing 1 μg of valine angiotensin II (Hypertensin, Ciba) and incubated, for 60 min, at 37℃. After incubation, the mixture was injected intravenously into another rabbit whose blood pressure was kymographically recorded. In activation of angiotensin II by the boiled anddialyzed extract was further investigated. The effect of EDTA addition during the inactivation process was also studied. The Sephadex Gelfiltration technique was applied to the renal extract before and after the incubation with AT II to find the active substances infractions or to recover remaining angiotensin in that mixture. The results of the above experiments were as follows: 1) By injection of 1 μg of AT II, blood pressure of normal rabbit was temporarily elevated 24.6 ± 4.35 mmHg above the basal blood pressure, and its pressor activity was not affected by incubation at 37℃ for 1 hour. 2) The pressor activity of AT II was markedly inhibited by incubation with renal medullary extract. Cortical extract showed the same inhibitory activity but occasionally its results became not clear due to the presence of renin-like substance in the extract. 3) AT II inhibitory activity of medullary or cortical extract was lost completely by boiling. 4) AT II inhibiting substance in the renal extract was undialysable and stable at pH 5.8-7.4 for at least 24 hours. The inhibitory activity was not affected by addition of EDTA during incubation which means that the extract in activated AT II independently to the calcium ion.
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腎性高血圧症についての研究 : 第6報腎 Angiotensinase についての検討

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腎性高血圧症についての研究 : 第6報 腎 Angiotensinase についての検討

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腎性高血圧症についての研究 : 第6報腎 Angiotensinase についての検討