精管切除の影響についての研究 : 第3報 精管切除ラットおよび人の精巣についての電子顕微鏡的研究
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Ultra structural studies of the testes of the adult rats and men after vasectomy were performed. The rats were killed at intervals ranging from 2 weeks to 3 months after vasectomy, and small pieces of their testes dissected from each animal were fixed in 2 % osmium tetroxide or 3 % potassium permanganate buffered with veronal acetate to pH 7.4. The biopsied materials of testes from 29 to 36-year-old men who had been vasectomized for sterility 3 to 7 years ago, were also examined in the present experiment. The results were summarized as follows: 1) Cells bearing a close resembrance to smooth muscle cells, and fibrocytes were observed in the boundary tissue of seminiferous tubules in both the normal rats and men. In the rats there existed one layer of the smooth muscle-like cells and in the men 4 layers of them in their boundary tissue of seminiferous tubules, where no significant structural changes were detected after vasectomy. 2) No histological differences were recognized in the process of spermatogenesis among the rats with and without vasectomy, while the spermatids of vasectomized men showed degenerative features, to some extent. They were mainly manifested by discontinuity of the nuclear membrane, widening of the subacrosomal space, appearance of many small vacuoles in the post nuclear region and abnormal formation of the acrosome. 3) There were no structural alterations in the interstitial cells of the vasectomized men and rats. 4) Many vacuoles of unidentifiable nature were found in the Sertoli cells. In rats and men, the effects of vasectomy on the process of spermatogenesis were discussed on the basis of the ultra fine structure.
- 社団法人日本泌尿器科学会の論文
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