泌尿器科領域におけるAndrogenの研究 : 尿中17-KS分画Testosterone Radioactivity

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For the purpose of the study on androgens in urological fields, urinary 17-ketosteroid fractions and Testosterone Radioactivity were determined by using the modified KANBEGAWA'S method (1967), and a few experimental studies on male urines were made. Urinary 17-Ketosteroids were fractionated into Dyhydroepiandrosterone (DHA), Androsterone (ANDRO), Etiocholanolone (ETIO), 11-β-hydroxy-androsterone (11-OXY-A), 11-β-p-hydroxy-etiocholanolone (11-OXY-E), 11-β-keto-androsterone (11-OXO-A), and 11-keto-etiocholanolone (11-OXO-E) by the use of gradient elution chromatography. In respect of Testosterone Radioactivity, after Testosterone-4-^<14>C injection, the position and ratio of Testosterone-4-^<14>C's radioactivity in urinary 17-Ketosteroid fractions were determined. The outline of procedure was as follows : After intravenous injection of Testosterone-4-^<14>C 1-1.2 μc (or Testosterone-4-^<14>C 1-1.2 μc and DHA-^8H 2-4μc), urine was collected for 3 days. Utilizing 200 ml of each of their 24 hour urine, the following technique was done. That is, urinary 17-Ketosteroid fractions were determined with β-gluculonidase hydrolysis, solvolysis, saponification, gradient elution chromatography, and spectrophotometry. All radioactive counting was done in a Packard Tri-Carb liquid scintillation spectrometer. The results of experimental studies were as follows : (A) Urinary 17-Ketosteroid fractions : 1) Prostatic cancer: In urinary 17-Ketosteroid fractions, the level of Etiocholanolone was much higher than Androsterone, then, the ANDRO-ETIO ratio was low. In the so-called adrenal fractions of urinary 17-Ketosteroid, DHA was low, but 11-keto-17KS and 11-β-hydroxy-17KS were high as compared with healthy male subjects.
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泌尿器科領域におけるAndrogenの研究 : 尿中17-KS分画Testosterone Radioactivity

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