前立腺の自己免疫抗原の研究

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It was shown that autoantibodies to the tissue of the rabbit prostate and the male accessory glands could be produced in the albino rabbits by intensive immunization with soluble protein of tissue preparation. Production of antibodies was detected by tanned cell hemagglutination or gel-diffusion technique. It was shown that antibodies produced reacted with tissue preparation from prostate and accessory glands, namely coagulating gland, bulbourethral glands and seminal vesicles. Two precipitation lines were seen on gel-diffusion plate, showing two reactive components in prostatic tissue preparation as reported by Shulman et al. No precipitation line was formed between serum and testis, kidney, liver, lung and pancreas. Chromatography on DEAE cellulose column separated tissue antigens in six fractions. 70% of total activity was recovered in the 2nd fractions. Disc electrophoresis on acrylamido-gel revealed six bands which located at the different site from that of acid phosphatase. However, precipitation band was visualized only faintly at the site of α^2-globulin and γ-globulin by immunoelectrophresis. Histlogical examination of the prostatic tissue from some immunized rabbits showed atrophy and eosinophilic stain reaction of epithelial cells suggesting diminished secretory activity. The direct immunofiuorescent technique performed on prostatic tissue revealed some fluorescent spots at epithelium.
社団法人日本泌尿器科学会の論文