健康者および不妊患者の射出精子におけるATPase活性の電顕的研究

概要

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The cytochemical localization and intensity of adenosine triphosphatase (ATPase) activity in the spermatozoa from fertile and infertile human ejaculate were observed by means of the electron microscope. Spermatozoa from fertile and infertile human ejaculate were fixed in 1% glutaraldehyde and treated histochemically to demonstrate Ca and Mg dependent ATPase. Furthermore, as substrates, ADP, AMP and β-glycerophosphate were used. The localization of Ca-activated ATPase was not different from that of Mg-activated ATPase. In the fertile human ejaculated spermatozoa, ATPase activity was found on the surface of the acrosome and mitochondria consisting of the mitochondrial sheath, around the outer coarse fibers and in the axial filament complex. Compared with the result with fertile specimen, in the infertile human ejaculated spermatozoa, ATPase activity on the motile structures, the outer coarse fibers and the axial filament complex were considerably weak and occasionally not recognized. From this study, it may be considered that ATPase around the outer coarse fibers and in the axial filament complex of spermatozoa may serve to mediate contraction of the axonemal elements during motility.
社団法人日本泌尿器科学会の論文