口腔領域より分離した尿素分解性 Proteus 菌に関する研究

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Distribution of variouss pecies of Proteus bacilli in oral area has been studied on a total of 1513 subjects including adults and children. Using S. S agar and also Endo's medium as subsidiary one for isolation of the organisms, a total of 133 strains of urea-decomposing Proteus bacilli were recovered from different sites of the mouth. These organisms consisted of, accorbing to Bergey's classification, 30 strains of P. vulgaris, 21 strains of P. mirabilis, 74 strains of P. morganii, and 8 strains of P. rettgeri. The organisms could be cultured from 8 cases of ulcerative pulpitis and acute pulpltis in 100%, from 8 cases of freshly extracted, uninfected tooth socket in 37.5%, from 9 cases of infected tooth socket in 33.3% and from 128 cases of debris as deposited inside artificial tooth crown which was temporarily held in position un-cemented, 32%. A definite seasonal variation in the recovery rate of the organisms was observed in that they were more abundantly grown during the months of April, May and June than during the wintertime. (Dic, Janu, Feb) Of all the species isolated Proteus morganii was most frequently met, occupying as much as 55% of the total isolates. General colonial appearance as observed on the culture media was as follows ; circular in shape with 1.5 to 5 mm in diameter, they were either flattened or slightly raised and were homogeneous in texture with smooth surface and periphery. In color they were either achromatic or pale yellow to yellow on E. M. B, T. S. I., Kligler and SS agar, pale pink or achromatic on Endo's and Christensen's media. It was characteristic of SS agar that a dark brown pigmentation occurred at the center of individual colonies of organis msproducing hydrogen sulfide in the medium and that rettgeri strains produced pale pink coloring there. The organisms were pleomorphic and, although greatly affected by age of culture, they were generally medium-siZed rods with stunt ends, measuring about 0.4-0.8μ×1.0-3.0μ. Of sugar fermenting reactions, it was especially noted that 23 out of 74 strains of Proteus morganii had the ability to ferment xylose. Development of the colonial swarming phenomenon was dependent upon the concentrations of agar in the medium used as well as upon the temperature applied. As to the swarming patterns, P. vulgaris and P. mirabilis assumed wave-like, and P, morganii and P. rettgeri assumed dendritic patterns. Apparently the former two speoies took swarming more reabily than the latter two. In P. mirabilis the bacterial film became tinged with bluish shade. Motility, flagellation and staining of the organisms were subject to the concentrations of medium and to the cultural temperature used, as alike the swarming phenomenon. Hemolysis was not observed on both human and rabbit bloods applying puncture inoculations on the plate. P. vurgalis. no pellicle was formed in bouillon by any member of the strains examined. Gelatin was liquefied in stratum only by P. vulgaris and P. mirabilis. Formation of hydrogen sulfide was observed in all strains of vulgaris and mirabilis and in 5 strains of morgaaii. General conclusions regarding the bacterial resistance against antibiotics as tested with "Sensitivity Tablets" from Roskilde Medical Company Ltd may be summarized as follows ; Streptomycin Chloromycetin Teramycin Streptothricin Auremmycin Penicillin.
九州歯科学会の論文

口腔領域より分離した尿素分解性 Proteus 菌に関する研究

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