Candida に関する研究 : 1. 厚膜胞子形成のための培地並びに培養条件に関する研究
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概要
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Candida albicans, which recently has provoked increasing attention due to its distinct human pathogenicity, permits differentiation from other members in the species of Candida most effectively by the chlamydospore formation. The author, therefore, has undertaken to study the culture media and the cultural conditions required for the optimum chlaydospore formation in this organism. The results are summarized as follows. 1) The optimum pH of the culture media ranged from 6.8 to 7.4, any pH outside of this range being unfavorable for the chlamydospore formation. The optimum temperature of culture was 25℃, the room temperature from 18℃ to 20℃ were suboptimum, and 37℃ was unfavorable. 2) It was recognized that successful chlamydospore formation depended largely upon previous cultural procedure adopted prior to transplanting the organism into the media for that purpose. One of the main cultural factors for good result is to select luxuriantly growing young culture of the organism for the transplantation. For this rejuvnating pre-culture, Sabouraud agar with 5% liver infusion was used most favorably, keeping the organism at the temperature of 37℃ for 24 to 48 hours. 3) Better result was obtained for the chlamydospore formation by applying "cover slip method", using 1% starch agar with 0.3% meat infusion at pH7.2 rather than by applying "slide culture method" using corn meal agar. By the "cover slip method" the chlamydospore appeared in the culture media in as short as 16 hours. It was shown that the starch in the media promoted development of the pseudomycelium and the chlamydospore formation, while the meat infusion was effective in promoting maturation of the chlamydospore to take its typical form. Cover slip method : Place one drop of saline suspension of Candida albicans on the middle of the plate agar over which a cover slip is laid flat. The petri dish was then covered ready to be incubated. Microscopic examination is recommended to be done through the cover slip. 4) Addition of congo red at the rate of 5microgram/cc to the culture media served to stain deep the thick membrane of the chlamydospore and aided in readily detecting the chlamydospore from among other confusing cells without giving any disadvantage for the development of the organism as well as for its chlamydospore formation.
- 九州歯科学会の論文
- 1958-06-30
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