感染根管並びに根端病巣より同時に分離した連鎖状球菌の生物学的性状及び抗生物質感受性に関する研究
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Bacteriological cultures were made from the infected root canals and apical foci of freshly extracted teeth which were diagnosed as carrying apical granuloma under clinical as well as radiographical observation. When strains of Streptococcus were recovered from these sources of a single teeth, these were comparatively studied with reference to the biological characteristics and antibiotics susceptibility. The data obtained were summarized as follows. 1) Isolation of the organism from infected root canals and apical foci was conducted on 81 teeth and the simultaneous occurrence of the organism in both sources was shown in 33 cases (40.7%). 2) It was especially noted during the cultural procedure that usually culture plates from the apical lesion were covered by bacterial film which rendered isolation of an organism from the plate something of difficulty. This was not the case with organisms involved in the root canal infection where isolation from the plate was readily accomplished. 3) Of 75 strains isolated, 33 came from infected root canals comprising 7 hemolytic streptococci and 26 nonhemolytic streptococci, and 42 came from apical foci comprising 15 hemolytic and 27 nonhemolytic streptococci. 4) Attempt to classify the isolates by examining the proposed hemolytic chracteristics confirmed the preaence of 17 strains of Type I, 2 atrains of Type II and one strain of Type III of hemolytic streptococci, according to Kobayashi's classification. There were 2 hemolytic streptococci which failed to conform to any type of the proposed characteristics. Most of the nonhemolytic streptococci were of α and α' types, and only 5 strains belonged to γ nonhemolytic type. 5) Based on the experiments on a total of 25 items of biological characteristics, all 75 strains of streptococci were classified into the following species, according to Bergey's classification : species number percentage reccovery Str. pyogenes 13 17.3% Str. equisimilis 4 5.3
- 九州歯科学会の論文
- 1960-05-31
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- 感染根管並びに根端病巣より同時に分離した連鎖状球菌の生物学的性状及び抗生物質感受性に関する研究