口腔のブドウ球菌 L-form に関する研究
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概要
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Recently research interest is being focused on the role of the L-form variety of Staphylococcus aureus derived from human mouth as to whether or not they were involved in the initiation and maintenance of chronic infections occurring in the oral region. Using the strain 131-1 of Staphylococcus aureus isolated from human mouth, we have carry undertaken to out a series of basic studies on the induction of organism to its L-form by exposure to Penicillin G or its reversion, and, utilizing the induced organisms, drug sensibility was examined, and, particularly, the cytopathological significance was studied with L-form organisms with use of tissue cell culture. Results obtained were summarized as follows. 1. Penicillin induction of L-form colonies of Staphylococcus aureus 131-1 was achieved in 3 to 4 days usiug the solid culture medium for L-form. 2. When the strain 131-1 of staphylococcus was grown in the L-form liquid medium for 4 to 5 days, proliferation of L-form colonies proceeded to the extent that they could be readily recognized by inspection. (the naked eye) 3. Experiments by minifiltration conducted on the growing L-form organisms in the liquid media confirmed that the minimum unit of bacterial growth size was below 0.45 micron. 4. The growth of the L-form organisms in the liquid media reached peak by 48 hours, which was slowed down with successive days. 5. Drug sensibility tests were carried out both for the strain 131-1 staphylococci and their L-form organisms, and the results showed that the latter showed higher resistance than the mother strain against some agents that inhibit the synthesis of bacterial cell wall. 6. The L-form organlsms could be recovered from the pus derived from chronic lesions in the mouth such as gingivitis and periodontitis in amount as much as 25%. 7. The L-form organisms were distributed 2-fold more abundantly in the pus from periodontitis lesions than from gingivitis lesions. 8. Observations on the possible cytopathic effect of the L-form organisms disseminated in the tissue cell culture revealed that 2 hours were sufficient to produce degeneration of the cell.
- 九州歯科学会の論文
- 1974-03-31